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Chapter 2: How We See the Invisible World

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Chapter 2: How We See the Invisible World

18 problems · 0 figures

2-1 §2.MC source ↗

Which of the following has the highest energy?
  1. a.
    light with a long wavelength
  2. b.
    light with an intermediate wavelength
  3. c.
    light with a short wavelength
  4. d.
    It is impossible to tell from the information given.

Answer

C. light with a short wavelength

2-2 §2.MC source ↗

You place a specimen under the microscope and notice that parts of the specimen begin to emit light immediately. These materials can be described as _____________.
  1. a.
    fluorescent
  2. b.
    phosphorescent
  3. c.
    transparent
  4. d.
    opaque

Answer

A. fluorescent

2-3 §2.MC source ↗

Who was the first to describe “cells” in dead cork tissue?
  1. a.
    Hans Janssen
  2. b.
    Zaccharias Janssen
  3. c.
    Antonie van Leeuwenhoek
  4. d.
    Robert Hooke

Answer

D. Robert Hooke

2-4 §2.MC source ↗

Who is the probable inventor of the compound microscope?
  1. a.
    Girolamo Fracastoro
  2. b.
    Zaccharias Janssen
  3. c.
    Antonie van Leeuwenhoek
  4. d.
    Robert Hooke

Answer

B. Zaccharias Janssen

2-5 §2.MC source ↗

Which would be the best choice for viewing internal structures of a living protist such as a Paramecium?
  1. a.
    a brightfield microscope with a stain
  2. b.
    a brightfield microscope without a stain
  3. c.
    a darkfield microscope
  4. d.
    a transmission electron microscope

Answer

C. a darkfield microscope

2-6 §2.MC source ↗

Which type of microscope is especially useful for viewing thick structures such as biofilms?
  1. a.
    a transmission electron microscope
  2. b.
    a scanning electron microscopes
  3. c.
    a phase-contrast microscope
  4. d.
    a confocal scanning laser microscope
  5. e.
    an atomic force microscope

Answer

D. a confocal scanning laser microscope

2-7 §2.MC source ↗

Which type of microscope would be the best choice for viewing very small surface structures of a cell?
  1. a.
    a transmission electron microscope
  2. b.
    a scanning electron microscope
  3. c.
    a brightfield microscope
  4. d.
    a darkfield microscope
  5. e.
    a phase-contrast microscope

Answer

B. a scanning electron microscope

2-8 §2.MC source ↗

What type of microscope uses an annular stop?
  1. a.
    a transmission electron microscope
  2. b.
    a scanning electron microscope
  3. c.
    a brightfield microscope
  4. d.
    a darkfield microscope
  5. e.
    a phase-contrast microscope

Answer

E. a phase-contrast microscope

2-9 §2.MC source ↗

What type of microscope uses a cone of light so that light only hits the specimen indirectly, producing a light image on a dark background?
  1. a.
    a transmission electron microscope
  2. b.
    a scanning electron microscope
  3. c.
    a brightfield microscope
  4. d.
    a darkfield microscope
  5. e.
    a phase-contrast microscope

Answer

D. a darkfield microscope

2-10 §2.MC source ↗

What mordant is used in Gram staining?
  1. a.
    crystal violet
  2. b.
    safranin
  3. c.
    acid-alcohol
  4. d.
    iodine

Answer

D. iodine

2-11 §2.MC source ↗

What is one difference between specimen preparation for a transmission electron microscope (TEM) and preparation for a scanning electron microscope (SEM)?
  1. a.
    Only the TEM specimen requires sputter coating.
  2. b.
    Only the SEM specimen requires sputter-coating.
  3. c.
    Only the TEM specimen must be dehydrated.
  4. d.
    Only the SEM specimen must be dehydrated.

Answer

B. Only the SEM specimen requires sputter-coating.

2-12 §2.FITB source ↗

When you see light bend as it moves from air into water, you are observing _________.

Answer

refraction

2-13 §2.FITB source ↗

A microscope that uses multiple lenses is called a _________ microscope.

Answer

compound

2-14 §2.FITB source ↗

Chromophores that absorb and then emit light are called __________.

Answer

fluorochromes

2-15 §2.FITB source ↗

In a(n) _______ microscope, a probe located just above the specimen moves up and down in response to forces between the atoms and the tip of the probe.

Answer

atomic force microscope

2-16 §2.FITB source ↗

What is the total magnification of a specimen that is being viewed with a standard ocular lens and a 40⨯ objective lens?

Answer

400⨯

2-17 §2.FITB source ↗

Ziehl-Neelsen staining, a type of _______ staining, is diagnostic for Mycobacterium tuberculosis.

Answer

acid-fast

2-18 §2.FITB source ↗

The _______ is used to differentiate bacterial cells based on the components of their cell walls.

Answer

Gram stain

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Problems and answers are adapted from Microbiology by Nina Parker, Mark Schneegurt, Anh-Hue Thi Tu, Philip Lister and Brian M. Forster, OpenStax, and are used under the CC BY-NC-SA 4.0 licence. Access for free at https://openstax.org/books/microbiology/pages/1-introduction. Illustrations: Copyright Rice University, OpenStax, under CC BY-NC-SA 4.0 license. This adaptation is released under the same licence, is non-commercial, and is neither affiliated with nor endorsed by OpenStax or Rice University.